Annotated gene sequence

Experiment = in vivo icSHAPE / NAI-N3 RefSeq = hg38 Cell = HEK293T Original Data Reference = Cell. 2016;165(5):1267-1279.

stable secondary structure loop bulge/internal loop no SHAPE data coding sequence

GCGACTGCGCAGCGTGGCCCAGCGCGGTCAAATTATAATACATAAAAGTTGTCAGGGCGGAGAGCAAGACATTACTCTTCTCGGATTGCCGGTTCGCTCG
CGAGACTTGAGCGTTGCTAGGAGATTCGGCAGGCGGGCGGAGCCAGACTCGGCGGGGCGGGGAGGGGTGGGGCTAGGCTCGGCGAGGCGAGGAAGGGTGG
GTGGAGCCAGGCTTGGCGGGCTGTGCGTGCTCGCGGTGGGCGGTGGCGGCGGCTGCCTCGCGAAGGTTCGAGATCCGTCGCGTGCGGGAGGCGGGCCGCG
ATCTTGCGCAGGGTCGGTGTGGGCGCAGGCTGCAGCGCCGCGACTCGTGCGGGTAGGCGTCTGCGCTCGGTTTGAGGGCTCGGCGCGGGGTTTCCTGTTC
CTCCTTCTGCGCGGCTGCAGCTCGGGACTTCGGCCTGACCCAGCCCCCATGGCTTCAGAAGAGCTACAGAAAGATCTAGAAGAGGTAAAGGTGTTGCTGG
AAAAGGCTACTAGGAAAAGAGTACGTGATGCCCTTACAGCTGAAAAATCCAAGATTGAGACAGAAATCAAGAACAAGATGCAACAGAAATCACAGAAGAA
AGCAGAACTTCTTGATAATGAAAAACCAGCTGCTGTGGTTGCTCCCATTACAACGGGCTATACGGTGAAAATCAGTAATTATGGATGGGATCAGTCAGAT
AAGTTTGTGAAAATCTACATTACCTTAACTGGAGTTCATCAAGTTCCCACTGAGAATGTGCAGGTGCATTTCACAGAGAGGTCATTTGATCTTTTGGTAA
AGAATCTAAATGGGAAGAGTTACTCCATGATTGTGAACAATCTCTTGAAACCCATCTCTGTGGAAGGCAGTTCAAAAAAAGTCAAGACTGATACAGTTCT
TATATTGTGTAGAAAGAAAGTGGAAAACACAAGGTGGGATTACCTGACCCAGGTTGAAAAGGAGTGCAAAGAAAAAGAGAAGCCCTCCTATGACACTGAA
ACAGATCCTAGTGAGGGATTGATGAATGTTCTAAAGAAAATTTATGAAGATGGAGACGATGATATGAAGCGAACCATTAATAAAGCCTGGGTGGAATCAA
GAGAGAAGCAAGCCAAAGGAGACACGGAATTTTGAGACTTTAAAGTCGTTTTGGGAACTGTGATGTGATGTGGAAATACTGATGTTTCCAGTAAGGGAAT
ATTGGTGAGCTGCATATATAAATTTGACAGATAGCTATTTACATAGCCTTCTAAGTAAAGGCAATGAATTCTCCATTTCCTACTGGAGGATTTATTTAAA
TAAAATATGCTTATTAAACACTCCTGCAAAGATGGTTTTATTAGTACCCTGGTCATTTTGTTCAAGGAAGGGTTATATTGCATTCTCACGTGAAATATAA
AAAGCAAGTCTTGCCCAATAAAAACGCTACATTGTGTGTATTTTTTGTTCAGCTAAGAATTGGAAAAGTATTTGCTTGCCTTTTAAGTTACTGACATCAG
CTTCCACCAGTGTAAAAATTGAGTAAAACCTGAAGTTTTGCATAAAATGCAAATCGGTGCCTGTGCTTGAAGGTTGCTGTAGAGCATCTGACCCCTTATT
ACCACCTTAAGCAATGTATATGCCATGCATTACCATGCACTAATTCAATCACAGGTGTTTCTATCTAGATTTAAATATATTTGTCAATGAATGTGGAATA
GAAAATCTAAACATGACAATAATAGACATATCTTTGTATGGTACCAGTTAGTTTTGCCGTGGATCAGATGGTTTATAAAAGTAATAACCATAAAGCAAAA
AATAATTTGAAAGCCCGTCTATTCCTATGCTCAATAAAGTTAAGTTTTTCTTCATTAGAACAGTTTTATGATTTATTTGTCTAGGAGTATGTCAGAAAAA
TCAGGCTTTTAGTAGGAATTACTCCTATTCCCCCTGAAGTCAGGACCAGTGCCTGTGATCTCCATTACTTTATTTTCCTGGAGGTATTAGCCAACACAGT
TAGATCAGAGAAAGCAATTGAAGCCAGGCATGAAGGCTGGCGCCCGTAATCCCAGCTACTAAGGCTGGAGGATCACTTCAGCCCAGGAGTTTAAGGCTGC
AGTGAGCTATGATGATGCCACTGTACTGCAGCTTGGGTAACAGATTGAGAACCTGTCTCATTAAAAAAAAAAAAAAAAAAAAAGCCGTTAGACACACAGG
AAAAATCCAGAAGGGTAAACTAAACTAAAGCTACAATTAATATGGGAATTTGGAAGAAGTGGTAGGATTTAAAATACAGAAACAGTTTATGTATAGGATA
GCTATAAGTAAATACTGAAACACATTATGCCTCTGTAATTGGGGTTGACACATGAACAGAATAGCAGACACAATGCATATGAAAGTTACAGAATATGGTA
AAAGTGGGGTAAAGATGGGTTTTTAATGATACTAAGATAACTGAAAATAGGCATATAGATATATTCCAAGCCGCCTGACGATCTAATTGTAAAAAGTAAA
GCATACAAATACTAGAAGAAAATGGAGGAAAACGACATTATATGTGACTTAAAACCTAGAAGAAATAAATAAAACTATTGATCAATTTTAACTACATAAA
AATGTTTATAGGACAAGAAAAACCCCACCATAACCCAAGGCAAACAATGTATTGACAGGATTCCAATAATTAAGAATACTTCATACAAAAAGAAATGTAA
ATGACCTTTAACATGTAAAGATGCTCACCTTGTTCAGAAGAGAATAAACCAGTGTTTTTACCTTTC

Loop counts:

3 nts: 3
4 nts: 1
5 nts: 2
6 nts: 1
7 nts: 2
8 nts: 1

Tetra-loop variants:

UAUU    1

Single bulge variants:

G x 0 bulge: 1
A x 0 bulge: 0
U x 0 bulge: 1
C x 0 bulge: 0

1 x 1 internal loop variants:

G x G bulge: 0
G x A bulge: 0
G x U bulge: 0
G x C bulge: 0
A x G bulge: 0
A x A bulge: 0
A x U bulge: 0
A x C bulge: 0
U x G bulge: 0
U x A bulge: 0
U x U bulge: 0
U x C bulge: 0
C x G bulge: 0
C x A bulge: 0
C x U bulge: 0
C x C bulge: 0