Annotated gene sequence

Experiment = in vivo icSHAPE / NAI-N3 RefSeq = hg38 Cell = HEK293T Original Data Reference = Cell. 2016;165(5):1267-1279.

stable secondary structure loop bulge/internal loop no SHAPE data coding sequence

AGTTTCCCGCCCAGAGGCAAGGGCGTGCGAGGGGATCCAGATATCCAAGGACCTGAGGTTTCGGCCTCGAGGTCTTGGGCGGGGGACTGGGCAGGCTGCG
CGGGGTCCCAGCGAGGGGACAGCTCGGGTGGGCGGCCAGGGTGTTGGGGGCTGCGGGCGGCGGACAAAGCGGCGGCACCACCCCGCGGCGCGGGCCAATG
GAATGAATGGGCTATAAATAGCCGCCAATGGGCGGCCCGCGTTGTGCCCCTTAAGAGCCGCGGGAGCGCGGAGCGGCCGCTGTTCGCCTGCGTCGCTCCG
GGAGCTGCCGACGGACGGAGCGCCCCCGCCCCCGCCCGGCCGCCCGCCCGCCGCCGCCATGCCCTTCTCCAACAGCCACAACGCACTGAAGCTGCGCTTC
CCGGCCGAGGACGAGTTCCCCGACCTGAGCGCCCACAACAACCACATGGCCAAGGTGCTGACCCCCGAGCTGTACGCGGAGCTGCGCGCCAAGAGCACGC
CGAGCGGCTTCACGCTGGACGACGTCATCCAGACAGGCGTGGACAACCCGGGCCACCCGTACATCATGACCGTGGGCTGCGTGGCGGGCGACGAGGAGTC
CTACGAAGTGTTCAAGGATCTCTTCGACCCCATCATCGAGGACCGGCACGGCGGCTACAAGCCCAGCGATGAGCACAAGACCGACCTCAACCCCGACAAC
CTGCAGGGCGGCGACGACCTGGACCCCAACTACGTGCTGAGCTCGCGGGTGCGCACGGGCCGCAGCATCCGTGGCTTCTGCCTCCCCCCGCACTGCAGCC
GCGGGGAGCGCCGCGCCATCGAGAAGCTCGCGGTGGAAGCCCTGTCCAGCCTGGACGGCGACCTGGCGGGCCGATACTACGCGCTCAAGAGCATGACGGA
GGCGGAGCAGCAGCAGCTCATCGACGACCACTTCCTCTTCGACAAGCCCGTGTCGCCCCTGCTGCTGGCCTCGGGCATGGCCCGCGACTGGCCCGACGCC
CGCGGTATCTGGCACAATGACAATAAGACCTTCCTGGTGTGGGTCAACGAGGAGGACCACCTGCGGGTCATCTCCATGCAGAAGGGGGGCAACATGAAGG
AGGTGTTCACCCGCTTCTGCACCGGCCTCACCCAGATTGAAACTCTCTTCAAGTCTAAGGACTATGAGTTCATGTGGAACCCTCACCTGGGCTACATCCT
CACCTGCCCATCCAACCTGGGCACCGGGCTGCGGGCAGGTGTGCATATCAAGCTGCCCAACCTGGGCAAGCATGAGAAGTTCTCGGAGGTGCTTAAGCGG
CTGCGACTTCAGAAGCGAGGCACAGGCGGTGTGGACACGGCTGCGGTGGGCGGGGTCTTCGACGTCTCCAACGCTGACCGCCTGGGCTTCTCAGAGGTGG
AGCTGGTGCAGATGGTGGTGGACGGAGTGAAGCTGCTCATCGAGATGGAGCAGCGGCTGGAGCAGGGCCAGGCCATCGACGACCTCATGCCTGCCCAGAA
ATGAAGCCCGGCCCACACCCGACACCAGCCCTGCTGCTTCCTAACTTATTGCCTGGGCAGTGCCCACCATGCACCCCTGATGTTCGCCGTCTGGCGAGCC
CTTAGCCTTGCTGTAGAGACTTCCGTCACCCTTGGTAGAGTTTATTTTTTTGATGGCTAAGATACTGCTGATGCTGAAATAAACTAGGGTTTTGGCCTGC
CTGCGTCTG

Loop counts:

3 nts: 7
4 nts: 2
5 nts: 3
6 nts: 1
8 nts: 1
9 nts: 1

Tetra-loop variants:

UGGA    1
GUCU    1

Single bulge variants:

G x 0 bulge: 0
A x 0 bulge: 1
U x 0 bulge: 1
C x 0 bulge: 2

1 x 1 internal loop variants:

G x G bulge: 2
G x A bulge: 0
G x U bulge: 0
G x C bulge: 0
A x G bulge: 0
A x A bulge: 0
A x U bulge: 0
A x C bulge: 3
U x G bulge: 0
U x A bulge: 0
U x U bulge: 0
U x C bulge: 1
C x G bulge: 0
C x A bulge: 3
C x U bulge: 1
C x C bulge: 0