Annotated gene sequence

Experiment = in vivo icSHAPE / NAI-N3 RefSeq = hg38 Cell = HEK293T Original Data Reference = Cell. 2016;165(5):1267-1279.

stable secondary structure loop bulge/internal loop no SHAPE data coding sequence

GAAATCAGACTTTGGAGCCACAAAGAACCATTCCAATTCTGGGTCGTCCGCTTACACACGGTGAACGGAGCACATTTCTTCTCTGAGCCTTGGTTCCTTT
CCTGTAAATGGAGTATAACCACTCCCATCTTGCAGGAGCTTTTTGGTGATAATTAAGGTGACTTCGTGAAAAGTACCAGGGTCTCAAAAATCTCAAAATT
TGTGGAAAGTATGCAGTTTCTAGGTTGGAGAGGCTAGAGTTTGGTTTCAAAAGCCTCGACCACACACAGGGGCGGTTACAGCTCTTGAGGTCCCGGGGGT
CACAGGACGCCTTCCGAGTTGGGGGGAGGAGAAAACAAAACCACAGGAAGAAACGAACAACGCTTTCGGGTGGGCCCGGGACCATAGAGGGTCGCTCCGC
CACTTTCCTAGAGCGACCGCGGCGGCGCCAGAGCGCGGCGAGGCCCTCACTTCCGGCGGCGCGGGAGGCGCCCAGCGAGCCAGAGTGGTGGCTGGTCCCG
CGCGAAAATTCTGAGCTGTACACCTCTAGGAAATGAAACACTAGTTCAGAAGAAGCCTGTAAACTCTCTTACAAATACATTTGGTTATTCACCATGAGGT
TAGCAAAGCCTAAAGCGGGTATTTCTCGGAGCTCAAGCCAAGGAAAGGCCTATGAGAACAAGCGCAAAACAGGCCGGCAGCGGCAGAAGTGGGGCATGAC
TATTCGATTTGACTCAAGCTTCAGTAGACTCAGAAGAAGCTTGGATGACAAACCCTATAAATGTACTGAATGTGAAAAGAGTTTCAGTCAGAGTTCAACT
CTTTTTCAACACCAGAAGATCCATACTGGAAAGAAATCCCATAAATGTGCTGATTGTGGGAAAAGTTTCTTTCAGAGTTCTAATCTCATTCAGCATCGAC
GGATCCATACGGGGGAAAAGCCCTACAAATGTGATGAGTGTGGAGAAAGCTTCAAACAGAGCTCAAATCTCATTCAGCACCAGAGAATTCATACTGGAGA
AAAACCCTATCAGTGTGATGAGTGTGGCCGGTGTTTCAGCCAGAGCTCCCACCTTATTCAACATCAGAGAACCCACACTGGGGAGAAACCCTACCAGTGC
AGTGAATGTGGCAAATGTTTCAGTCAGAGCTCTCATCTGAGGCAGCACATGAAGGTGCATAAAGAAGAGAAGCCTCGTAAAACCCGGGGCAAAAATATCA
GGGTGAAGACTCACTTACCCTCTTGGAAAGCTGGTACAGGAAGGAAGTCTGTGGCTGGTCTCCGTTAAGTATAGGGCTTTTTGACAGCTTTTTGAGACCT
CTTAAGAAAAAATAAAAAGTAAAAAATGAAAGGAATCTTTTTTAGAAATAGAGATGCTTTATAGTAGATCACTTAAATACTGGATCTTTTGCTAGTGTGA
AAACATTGGGAATTTAATGACATTATTGAGCTGAAAGAAATTACATGAGTCCAGCTACCCTCATTTCTTTTTTATGTGACATGGAGCACAAAGAACTGAA
AATATGTTTTGAGGGAGCATGACATCCTTGACCTCATTTGAAATTACTTCCATTTTCAAAAACCATCATGTTTTGTAGATACATTTTGAGAAAAACCATC
ATGTTTTGAGGATACATTTGTGAAAGTGCAGGCATGCCAATGACTACTCAGTTTTAGGACTTTCTGTGGAAGAAAAAAGGGAGAAGAAATCAATTCCACT
ACTTTTGAAGTTCTGCAACAGATGAGATTTTGTTTGTAAAGTTTTGTAGTATATTAACCATTGGTTTATCACTCTAGATTCAACATATTAAAATGTATTC
AAGCTCACAGATTTTTAATCAGTAAGGCCTATCTATATTAGTTGTCTTTTATTTCTTCTCCTTGTGGACAGTTGTTAATGAAAGGAGTAAGGATTTCCCT
TTTTTTGTTTTGTTTGTTTTGTTTTTTTTAACCAAATTCTTAGAGATACTATAGAATCCAAATGAGAACTGAATTGGACCTCAAGTCTTCTATTCCTACT
AATAGAGTTCTTTGTGATGGTAACTGCTGTGTCGTTTGTTTTCCACAAGTTGGGATGGATTCATGTCGATACATCCCCATGCCCTTGACCTCTTCTGGCA
TTCTCCTGTGCTCTGACAAACTGAGCCAGCCTTTTAGATCTACATGAATAAACAAACTATTTTACCAAGAAAAATCTCAGCTTGCTTACTGCTTAATTAA
AAACCTACAATTTACACACCTCCCTGCCTTCAAGACTGTGAGCTTTGGATAGCCCTGCTTAAATGTTTGTCAACAACAAGAGTCGTAATGTGTCAGTACA
TTGTAGCTCCTTTGAAATTTAACTCTTTCTGTGTTACATGAATTGTTTTAAGGAGTCCGGCAAACATGTTTCTTCACTTCCATGAGAATGGTGCCAAGTG
TCAGACTCTAATGAGCCCTCAGCTCAGGTTTTAATTTCTATTGAATGCTAACATTCTTCTGATTTTTTGGTATCTTTTATAATCATATCGGTTTCTGCTG
TATTAATGACAATTATTCATGAAAATAAAAAATGAAATATTTTGTATACTA

Loop counts:

3 nts: 1
4 nts: 7
5 nts: 3
6 nts: 2
7 nts: 2
8 nts: 2
9 nts: 1

Tetra-loop variants:

GAUU    1
AGUU    1
UCAG    1
CUAA    1
UUCU    1
GAAA    1
ACAU    1

Single bulge variants:

G x 0 bulge: 0
A x 0 bulge: 1
U x 0 bulge: 0
C x 0 bulge: 0

1 x 1 internal loop variants:

G x G bulge: 0
G x A bulge: 0
G x U bulge: 0
G x C bulge: 0
A x G bulge: 0
A x A bulge: 0
A x U bulge: 0
A x C bulge: 0
U x G bulge: 0
U x A bulge: 0
U x U bulge: 0
U x C bulge: 0
C x G bulge: 0
C x A bulge: 0
C x U bulge: 0
C x C bulge: 0