Annotated gene sequence

Experiment = in vitro icSHAPE / NAI-N3 RefSeq = hg38 Cell = HEK293T Original Data Reference = Cell. 2016;165(5):1267-1279.

stable secondary structure loop bulge/internal loop no SHAPE data coding sequence

TGGGAGTACAGCTCTTTCCTTTCCGTCTGGCGGCAGCCATCAGGTAGGCTGCGTTGAGGATCTTTGCTCTTCCATCCGCCTTTGATCGTCTTCCTCTTCA
GCCATCCAGGTAAGCCAAGATGGGTGCATACAAGTACATCCAGGAGCTATGGAGAAAGAAGCAGTCTGATGTCATGCGCTTTCTTCTGAGGGTCCGCTGC
TGGCAGTACCGCCAGCTCTCTGCTCTCCACAGGGCTCCCCGCCCCACCCGGCCTGATAAAGCGCGCCGACTGGGCTACAAGGCCAAGCAAGGTTACGTTA
TATATAGGATTCGTGTTCGCCGTGGTGGCCGAAAACGCCCAGTTCCTAAGGGTGCAACTTACGGCAAGCCTGTCCATCATGGTGTTAACCAGCTAAAGTT
TGCTCGAAGCCTTCAGTCCGTTGCAGAGGAGCGAGCTGGACGCCACTGTGGGGCTCTGAGAGTCCTGAATTCTTACTGGGTTGGTGAAGATTCCACATAC
AAATTTTTTGAGGTTATCCTCATTGATCCATTCCATAAAGCTATCAGAAGAAATCCTGACACCCAGTGGATCACCAAACCAGTCCACAAGCACAGGGAGA
TGCGTGGGCTGACATCTGCAGGCCGAAAGAGCCGTGGCCTTGGAAAGGGCCACAAGTTCCACCACACTATTGGTGGCTCTCGCCGGGCAGCTTGGAGAAG
GCGCAATACTCTCCAGCTCCACCGTTACCGCTAATATAAGTAAAGTTTGTAAAATTCATACTTAATAAACAATTTAGGACAGTCATGTCTGCTTACAGGT
GTTATTTGTCTGTTAAAACTAGTCTGCAGATGTTTCTTGAATGCTTTGTCAAATTAAGAAAGTTAAAGTGCAATAATGTTTGAAGACAATAAGTGGTGGT
GTATCTTGTTTCTAATAAGATAAACTTTTTTGTCTTTGCTTTATCTTATTAGGGAGTTGTATGTCAGTGTATAAAACATACTGTGTGGTATAACAGGCTT
AATAAATTCTTTAAAAGGAGAGAACTGAAACTAGCCCTGTAGATTTGTCTGGTGCATGTGATGAAACCTGCAGCTTTATCGGAGTGATGGCAATGCTCTG
CTGGTTTATTTTCAAGTGGCTGCGTTTTTTTTAGTTTGGCAGGTGTAGACTTTTTAAGTTGGGCTTTAGAAAATCTGGGTTAGCCTGAAGAAAATTGCCT
CAGCCTCCACAGTACCATTTTAAATTCACATAAAAGGTGAAAGCTCCTGGTTCAGTGCCATGGCTTCATGGCATTCAGTGATTAGTGGTAATGGTAAACA
CTGGTGTGTTTTGAAGTTGAATGTGCGATAAAATTATTAGCCTTAAGATTGGTAAGCTAGCAATGAATGCTAGGGTGGGAAGCTGGTGAGCCAGTGGCCA
TTAGATAAATACCTTTCAAGTGTGAGCTTAGACGTCAACCCTAAAATACTTAACCGTAATGCTAATTGTGATCATTATGAATCCCTTCAGTCACATTAGG
GGGAAAGTAGTTGGCTATAAGTACGTCATTCTTAGTCCAGTCAGTCTTAAAAACATCTTGGGTTACCCACTCTGTCCACTCCCATAGGCTACAGAAAAAG
TCACAAGCGCATGGTTTCCAACCATATGTGTTTTCTGCAGTTATTTCTCTTGTTCTGGCCAAACAACCCTAAAAATCCTTACCATTCCACAAAGTTGGAC
CATCACTTGTGCACCCACTTTGACTATGAGTATACCACCACATTGCATTTCTGTTTGCACCATGTCTTCCAGGAGACTAGACTACTGTTGTCCAGGGTCA
ATTTGAGTGTAAAGAAAATGTAGACAAGGAATTGCCCAATTTTAAATTCTGACTTTGCTGACTTAATTTAAATGCTCGTTCTGAACCAATTTTCTCCTAT
CTTCTCTAGGGGTTTCAAAAGACTCAGTTAATTGATTTCCAGGAAGTACTCATAGCAAGTTCATAAAAGTTCTTGAGACCTAAATTTCTTCACAAAAAAA
GAAAAGATCTTAAGTCATACATTTTAATTGTGTAGAGGTTGTTCAACTGAAGG

Loop counts:

3 nts: 2
4 nts: 4
6 nts: 2
7 nts: 2
8 nts: 3

Tetra-loop variants:

GCUU    1
UGGU    1
GGAA    1
CUUA    1

Single bulge variants:

G x 0 bulge: 0
A x 0 bulge: 1
U x 0 bulge: 0
C x 0 bulge: 0

1 x 1 internal loop variants:

G x G bulge: 0
G x A bulge: 0
G x U bulge: 0
G x C bulge: 0
A x G bulge: 0
A x A bulge: 0
A x U bulge: 0
A x C bulge: 1
U x G bulge: 0
U x A bulge: 0
U x U bulge: 0
U x C bulge: 0
C x G bulge: 0
C x A bulge: 1
C x U bulge: 0
C x C bulge: 0