Annotated gene sequence

Experiment = in vitro icSHAPE / NAI-N3 RefSeq = hg38 Cell = HEK293T Original Data Reference = Cell. 2016;165(5):1267-1279.

stable secondary structure loop bulge/internal loop no SHAPE data coding sequence

CCCCGCCCCCGCCGCCCCAGTCCCCGCCCCTCGCGCCTTCGTTTATTCCTCCGCGCGCTGGGACAGGCTGCTTCTTCGCCAGAACCAACCGGTTGCTTGC
TGTCCCAGCGGCGCCCCCTCATCACCGTCGCCATGCCCGGAGGTCTGCTTCTCGGGGACGTGGCTCCCAACTTTGAGGCCAATACCACCGTCGGCCGCAT
CCGTTTCCACGACTTTCTGGGAGACTCATGGGGCATTCTCTTCTCCCACCCTCGGGACTTTACCCCAGTGTGCACCACAGAGCTTGGCAGAGCTGCAAAG
CTGGCACCAGAATTTGCCAAGAGGAATGTTAAGTTGATTGCCCTTTCAATAGACAGTGTTGAGGACCATCTTGCCTGGAGCAAGGATATCAATGCTTACA
ATTGTGAAGAGCCCACAGAAAAGTTACCTTTTCCCATCATCGATGATAGGAATCGGGAGCTTGCCATCCTGTTGGGCATGCTGGATCCAGCAGAGAAGGA
TGAAAAGGGCATGCCTGTGACAGCTCGTGTGGTGTTTGTTTTTGGTCCTGATAAGAAGCTGAAGCTGTCTATCCTCTACCCAGCTACCACTGGCAGGAAC
TTTGATGAGATTCTCAGGGTAGTCATCTCTCTCCAGCTGACAGCAGAAAAAAGGGTTGCCACCCCAGTTGATTGGAAGGATGGGGATAGTGTGATGGTCC
TTCCAACCATCCCTGAAGAAGAAGCCAAAAAACTTTTCCCGAAAGGAGTCTTCACCAAAGAGCTCCCATCTGGCAAGAAATACCTCCGCTACACACCCCA
GCCTTAAGTCTCTTGGAGAAGCTGGTGCTGTGAGCCAGAGGATGTCAGCTGCCAATTGTGTTTTCCTGCAGCAATTCCATAAACACATCCTGGTGTCATC
ACAGCCAAGGTTTTTAGGTTGCTATACCAATGGCTTATTAAATGAAAATGGCACTAAAAGTTTCTTGAGATTCTTTATACTCTCTGCCTTCAGCAATCAA
TTCCATTCATACATCAGCACTCTGCTGGTTCTGTTTGAAATATGTTCTGTATTTAAAACTCAAATCTTGTTGGATCTCTGCAGGGCTTGTGACCAATGAA
GTCATATTTGTTGATGGTTGACAAAGCTTGCTTCACTCCATCAGAGAATGACTATCAATTTTTTTTTAACTGTCCTATCACGTCCTCTCCTGTCACCCAT
TTTGAAGAGTGGCAGAACTTGAAGTTCAACTTCCTCTGTAAATATCCAAGTATAAAGCCCAGGAACTTCTAGAATAACCCAGATGCGCTTTAATTTTTTT
TAATATGTTTTGATCACAGAACTTCTAGAATAACCCAGATGCTCTTTCATATTCTTTTAATACATCTTGATCACAGCTGGGGGAAAAAAAGCTTTTTAAT
TCTATACCTTCCTAGTAGATAAGTGAAGAGCAGGGAAAGAGACCTTTAAATATTTTGCTATAAAAAAATTTGTGATAAGTTTCTATCAAAATGGGGAGAT
TGCAGAAAAGGCTTCCCTTGGCTCCCAAGGAGGTGTAGCAGGTGTGAGCAATATTAGTGCCATGTGCCTTTCACACAGGGTTTGCATTTATCAGTCTGTT
TTCCGATGATGTGTACATGAAAGAGTACACCATGTGAAGAGAAGAGAGAATGATTGAAAATGTTTTAGTATAGAACTCTTCTTGCAGTGGGTTGCTATTT
TCTAGATTTTACTTTTTAGGGAACAAAATAAAATCCTTTGTTAAAACTGGG

Loop counts:

3 nts: 3
4 nts: 3
5 nts: 1
6 nts: 2

Tetra-loop variants:

UCGA    1
UGCC    1
GUUG    1

Single bulge variants:

G x 0 bulge: 0
A x 0 bulge: 3
U x 0 bulge: 0
C x 0 bulge: 0

1 x 1 internal loop variants:

G x G bulge: 1
G x A bulge: 0
G x U bulge: 0
G x C bulge: 0
A x G bulge: 0
A x A bulge: 0
A x U bulge: 0
A x C bulge: 1
U x G bulge: 0
U x A bulge: 0
U x U bulge: 0
U x C bulge: 0
C x G bulge: 0
C x A bulge: 1
C x U bulge: 0
C x C bulge: 0