Annotated gene sequence

Experiment = in vitro icSHAPE / NAI-N3 RefSeq = hg38 Cell = HEK293T Original Data Reference = Cell. 2016;165(5):1267-1279.

stable secondary structure loop bulge/internal loop no SHAPE data coding sequence

TCTGGGCGCGCGCGACGTCAGTTTGAGTTCTGTGTTCTCCCCGCCCGTGTCCCGCCCGACCCGCGCCCGCGATGCTGGCGCTGCGCTGCGGCTCCCGCTG
GCTCGGCCTGCTCTCCGTCCCGCGCTCCGTGCCGCTGCGCCTCCCCGCGGCCCGCGCCTGCAGCAAGGGCTCCGGCGACCCGTCCTCTTCCTCCTCCTCC
GGGAACCCGCTCGTGTACCTGGACGTGGACGCCAACGGGAAGCCGCTCGGCCGCGTGGTGCTGGAGCTGAAGGCAGATGTCGTCCCAAAGACAGCTGAGA
ACTTCAGAGCCCTGTGCACTGGTGAGAAGGGCTTCGGCTACAAAGGCTCCACCTTCCACAGGGTGATCCCTTCCTTCATGTGCCAGGCGGGCGACTTCAC
CAACCACAATGGCACAGGCGGGAAGTCCATCTACGGAAGCCGCTTTCCTGACGAGAACTTTACACTGAAGCACGTGGGGCCAGGTGTCCTGTCCATGGCT
AATGCTGGTCCTAACACCAACGGCTCCCAGTTCTTCATCTGCACCATAAAGACAGACTGGTTGGATGGCAAGCATGTTGTGTTCGGTCACGTCAAAGAGG
GCATGGACGTCGTGAAGAAAATAGAATCTTTCGGCTCTAAGAGTGGGAGGACATCCAAGAAGATTGTCATCACAGACTGTGGCCAGTTGAGCTAATCTGT
GGCCAGGGTGCTGGCATGGTGGCAGCTGCAAATGTCCATGCACCCAGGTGGCCGCGTTGGGCTGTCAGCCAAGGTGCCTGAAACGATACGTGTGCCCACT
CCACTGTCACAGTGTGCCTGAGGAAGGCTGCTAGGGATGTTAGACCTCGGCCAGGACCCACCACATTGCTTCCTAATACCCACCCTTCCTCACGACCTCA
TTTCTGGGCATCTTTGTGGACATGATGTCACCCACCCCTTGTCAAGCATTGCCTGTGATTGCCCAGCCCAGATTCATCTGTGCCTTGGACATGGTGATGG
TGATGGGTTGCCATCCAAGTGAAAGTCTTTTCCTTGACCAAGGGGGACAGTCAGTTTTGCAAAAGGACTCTAATACCTGTTTAATATTGTCTTCCTAATT
GGGATAATTTAATTAACAAGATTGACTAGAAGTGAAACTGCAACACTAACTTCCCCGTGCTGTGGTGTGACCTGAGTTGGTGACACAGGCCACAGACCCC
AGAGCTTGGCTTTTGAAACACAACTCAGGGCTTTTGTGAAGGTTCCCCCGCTGAGATCTTTCCTCCTGGTTACTGTGAAGCCTGTTGGTTTGCTGCTGTC
GTTTTTGAGGAGGGCCCATGGGGGTAGGAGCAGTTGAACCTGGGAACAAACCTCACTTGAGCTGTGCCTAGACAATGTGAATTCCTGTGTTGCTAACAGA
AGTGGCCTGTAAGCTCCTGTGCTCCGGAGGGAAGCATTTCCTGGTAGGCTTTGATTTTTCTGTGTGTTAAAGAAATTCAATCTACTCATGATGTGTTATG
CATAAAACATTTCTGGAACATGGATTTGTGTTCACCTTAAATGTGAAAATAAATCCTATTTTCTATGGAAGACTGGTACCTGGTTTCTGGAAGAGGGGTC
TGTGACTTGGAGCTGATCTTTACTGAGCTCGCCGTGGCAGATGCCATGCTCAGGACGTTCATGTGGATGGTTTCATGTCATCGTGCTGGCAACTTGTCCT
CCCTGCCTTAGAGATGAGGCTCAGACAAACGACCTTAGCACCCATAGCCTATGCCATGAGCACTGGCTCCACCCTGAATCCCAGCTCCTCCCCTTAGTGA
CCCCAAGTCTGTTTCCCTCAGCTGCATAAGGAGGCGATATAGTTTGAATATTTGTCCCCAGCCAAATCTCATGTTGAACTGTAATCCCCAGTGCTGGAGG
TGGGGCCTGCTACGAGGTGTTTGGATCATGGGGACGGGTATTTCATGGCTTGGTGCTGTTTTCTTGATGGTGAATTATTGCAAGATACGGTCATTTAAAA
TTGTGTGGCACCTCCCCCTGCCCCCTTCTTGCTCCTGCTTTCACCATGTGACATGCCTGATCCCCCTTCACCTTTTGCCATGGTCATAAGCTTCCTGAGG
CCTCCCTGGAAGCTGAGCAGATGCCAGCACCATGCTTCCTGTACATCCTGCAGAACCATAAGCCAATTAAACCTTTTTAATAATAAATTA

Loop counts:

4 nts: 6
7 nts: 2
9 nts: 2

Tetra-loop variants:

UUUC    1
UUCA    1
GUUG    1
GUCU    1
UGUU    1
ACUU    1

Single bulge variants:

G x 0 bulge: 0
A x 0 bulge: 1
U x 0 bulge: 0
C x 0 bulge: 1

1 x 1 internal loop variants:

G x G bulge: 0
G x A bulge: 2
G x U bulge: 0
G x C bulge: 0
A x G bulge: 2
A x A bulge: 0
A x U bulge: 0
A x C bulge: 0
U x G bulge: 0
U x A bulge: 0
U x U bulge: 0
U x C bulge: 1
C x G bulge: 0
C x A bulge: 0
C x U bulge: 1
C x C bulge: 0